Composite

Part:BBa_K4578008:Design

Designed by: Kelly Yoon   Group: iGEM23_USAFA   (2023-10-11)


INP-OspA expression block


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1038
    Illegal EcoRI site found at 1605
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1038
    Illegal EcoRI site found at 1605
    Illegal NheI site found at 151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1038
    Illegal EcoRI site found at 1605
    Illegal BglII site found at 1656
    Illegal BamHI site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1038
    Illegal EcoRI site found at 1605
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1038
    Illegal EcoRI site found at 1605
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 740
    Illegal AgeI site found at 594
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 769
    Illegal SapI.rc site found at 427


Design Notes

INP added


Source

doi

References